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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
21/09/2017 |
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Actualizado : |
09/10/2019 |
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Autor : |
SOUTO, E.P.F.; MAIA, L.A.; OLINDA, R.G.; GALIZA, G.J.N.; KOMMERS, G.D.; KOMMERS, G.D; MIRANDA-NETO, E.G.; RIET-CORREA, F. |
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Afiliación : |
E. P. F. SOUTO, Laboratory of Veterinary Pathology, Universidade Federal de Campina Grande, Patos, PB, Brasil.; L. A. MAIA, Laboratory of Veterinary Pathology, Universidade Federal de Campina Grande, Patos, PB, Brasil.; R. G. OLINDA, Laboratory of Veterinary Pathology, Universidade Federal de Campina Grande, Patos, PB, Brasil.; G. J. N. GALIZA, Laboratory of Veterinary Pathology, Universidade Federal de Campina Grande, Patos, PB, Brasil.; G. D. KOMMERS, Pathology Department,Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul and‡Medical Clinic for Large Animals, UniversidadeFederal de Campina Grande, Patos, PB, Brazil .; G. D. KOMMERS, Pathology Department,Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul and?Medical Clinic for Large Animals, UniversidadeFederal de Campina Grande, Patos, PB, Brazil .; E. G. MIRANDA-NETO, Pathology Department,Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul and?Medical Clinic for Large Animals, UniversidadeFederal de Campina Grande, Patos, PB, Brazil.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Laboratory of Veterinary Pathology, Universidade Federal de Campina Grande, Patos, PB,Brasil. |
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Título : |
Pythiosis in the nasal cavity of horses. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Journal of Comparative Pathology, v. 155, p. 126-129, 2016. |
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DOI : |
10.1016/j.jcpa.2016.06.005 |
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Idioma : |
Inglés |
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Contenido : |
Abstract.
Two cases of nasal pythiosis are reported in horses from the semi-arid region of northeastern Brazil. From received 830 equine samples, 156 (18.79%) of which were diagnosed with pythiosis. Of these, two horses (1.28%), a male and a female adult crossbreed , had lesions in the nasal cavity. Both horses had access to water reservoirs. Clinically, they had swelling in the rhinofacial region and a serosanguineous nasal discharge. Macroscopically, in case 1, the lesion affected the nasal vestibule, extending to the alar cartilage and nasal septum. In case 2, the lesion extended through the turbinates and the meatuses of the nasal cavity, as well as the ethmoid region. In both cases, the lesions were characterized by having a yellowegrey granular surface with cavitations of different sizes containing coral-like masses of necrotic tissue (kunkers). Histologically, multi-focal necrotizing eosinophilic rhinitis associated with hyphae (2e8 mm) similar to Pythium insidiosum were observed. In case 2, the lesions extended to the muscle, cartilage and bone adjacent to the nasal cavity and lungs. The diagnosis was confirmed by immunohistochemistry. It is concluded that nasal pythiosis occurs sporadically in horses in the semi-arid region of northeastern Brazil where cutaneous pythiosis is prevalent. |
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Palabras claves : |
HORSE; PHYTHIUM SPP; PLATAFORMA SALUD ANIMAL; RHINITIS. |
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Thesagro : |
CABALLOS; ENFERMEDADES DE LOS ANIMALES. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02108naa a2200289 a 4500
001 1057589
005 2019-10-09
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jcpa.2016.06.005$2DOI
100 1 $aSOUTO, E.P.F.
245 $aPythiosis in the nasal cavity of horses.$h[electronic resource]
260 $c2016
520 $aAbstract. Two cases of nasal pythiosis are reported in horses from the semi-arid region of northeastern Brazil. From received 830 equine samples, 156 (18.79%) of which were diagnosed with pythiosis. Of these, two horses (1.28%), a male and a female adult crossbreed , had lesions in the nasal cavity. Both horses had access to water reservoirs. Clinically, they had swelling in the rhinofacial region and a serosanguineous nasal discharge. Macroscopically, in case 1, the lesion affected the nasal vestibule, extending to the alar cartilage and nasal septum. In case 2, the lesion extended through the turbinates and the meatuses of the nasal cavity, as well as the ethmoid region. In both cases, the lesions were characterized by having a yellowegrey granular surface with cavitations of different sizes containing coral-like masses of necrotic tissue (kunkers). Histologically, multi-focal necrotizing eosinophilic rhinitis associated with hyphae (2e8 mm) similar to Pythium insidiosum were observed. In case 2, the lesions extended to the muscle, cartilage and bone adjacent to the nasal cavity and lungs. The diagnosis was confirmed by immunohistochemistry. It is concluded that nasal pythiosis occurs sporadically in horses in the semi-arid region of northeastern Brazil where cutaneous pythiosis is prevalent.
650 $aCABALLOS
650 $aENFERMEDADES DE LOS ANIMALES
653 $aHORSE
653 $aPHYTHIUM SPP
653 $aPLATAFORMA SALUD ANIMAL
653 $aRHINITIS
700 1 $aMAIA, L.A.
700 1 $aOLINDA, R.G.
700 1 $aGALIZA, G.J.N.
700 1 $aKOMMERS, G.D.
700 1 $aKOMMERS, G.D
700 1 $aMIRANDA-NETO, E.G.
700 1 $aRIET-CORREA, F.
773 $tJournal of Comparative Pathology$gv. 155, p. 126-129, 2016.
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Registro original : |
INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/06/2021 |
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Actualizado : |
25/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FEDERICI, M.; ARTIGAS, R.; GUERRA, S.; BRANDA, A.; VÁZQUEZ, N.; NICOLINI, P.; DUTRA, F.; LLAMBÍ, S. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODY ARTIGAS, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay; SOFÍA GUERRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NOELIA VÁZQUEZ, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay.; PAULA NICOLINI, Instituto Superior de la Carne, Universidad de la República (UDELAR), Tacuarembó, Uruguay.; FERNANDO DUTRA QUINTELA, División Laboratorios Veterinarios (DILAVE), Ministerio de Agricultura y Pesca (MGAP), Treinta y Tres, Uruguay.; SILVIA LLAMBÍ, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay. |
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Título : |
Detection of the Brachyspina mutation in Uruguayan Holstein cows using real time PCR and melting curve analysis. [Detecção da mutação da Brachyspina em vacas Holandês Uruguaias usando PCR em tempo real e análise da curva de fusão.] |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ciencia Rural, 2021, volume 51 (9), Article e20200872. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200872 |
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ISSN : |
1678-4596 (online) |
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DOI : |
10.1590/0103-8478cr20200872 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 17 Sept 2020; Accepted 05 Jan 2021; Reviewed 07 Mar 2021; Publication in this collection 02 June 2021; Date of issue 2021. |
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Contenido : |
ABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed.
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RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigotos normais e quatro heterozigotos foram detectados e confirmados através da análise tradicional de PCR e electophorese. Concluímos que o novo método é uma ferramenta confiável, altamente precisa e de baixo custo, que poderia ser usado para monitorar a presença da mutação BS na raça Holandês uruguaia. MenosABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed.
.-.-.-.-.-.-.-.-.-.-
RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigo... Presentar Todo |
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Palabras claves : |
Análise da curva de fusão; Bos taurus; Brachyspina syndrome; Holandês; Holstein; Melting curve analysis; PCR em tempo real; Real time PCR; Sindrome de Brachyspina. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://www.scielo.br/j/cr/a/kTxvzMF47Cqdb7TbPKHjxxc/?lang=en&format=pdf
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Marc : |
LEADER 03178naa a2200349 a 4500
001 1062156
005 2021-06-25
008 2021 bl uuuu u00u1 u #d
022 $a1678-4596 (online)
024 7 $a10.1590/0103-8478cr20200872$2DOI
100 1 $aFEDERICI, M.
245 $aDetection of the Brachyspina mutation in Uruguayan Holstein cows using real time PCR and melting curve analysis. [Detecção da mutação da Brachyspina em vacas Holandês Uruguaias usando PCR em tempo real e análise da curva de fusão.]$h[electronic resource]
260 $c2021
500 $aArticle history: Received 17 Sept 2020; Accepted 05 Jan 2021; Reviewed 07 Mar 2021; Publication in this collection 02 June 2021; Date of issue 2021.
520 $aABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed. .-.-.-.-.-.-.-.-.-.- RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigotos normais e quatro heterozigotos foram detectados e confirmados através da análise tradicional de PCR e electophorese. Concluímos que o novo método é uma ferramenta confiável, altamente precisa e de baixo custo, que poderia ser usado para monitorar a presença da mutação BS na raça Holandês uruguaia.
653 $aAnálise da curva de fusão
653 $aBos taurus
653 $aBrachyspina syndrome
653 $aHolandês
653 $aHolstein
653 $aMelting curve analysis
653 $aPCR em tempo real
653 $aReal time PCR
653 $aSindrome de Brachyspina
700 1 $aARTIGAS, R.
700 1 $aGUERRA, S.
700 1 $aBRANDA, A.
700 1 $aVÁZQUEZ, N.
700 1 $aNICOLINI, P.
700 1 $aDUTRA, F.
700 1 $aLLAMBÍ, S.
773 $tCiencia Rural, 2021, volume 51 (9), Article e20200872. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200872
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